This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p1.2-Hyg-Fur
catalog :
162774
citations: 1
Reference
Kovnir S, Orlova N, Shakhparonov M, Skryabin K, Gabibov A, Vorobiev I. A Highly Productive CHO Cell Line Secreting Human Blood Clotting Factor IX. Acta Naturae. 2018;10:51-65 pubmed
product information
Catalog Number :
162774
Product Name :
p1.2-Hyg-Fur
article :
doi
id28215509
pubmed_id29713519
bacterial resistance :
Ampicillin
cloning :
backbonep1.1
backbone_mutationThe region containing the EMCV IRES and the DHFR ORF from the p1.1 expression vector is removed. Antibiotic resistance genes and the SV40 promoter and terminator regions are added.
backbone_originLMBT, LTD
backbone_size11914
promoterCHO Elongation factor 1A
sequencing_primer_3SQ-3CH1-R ACAAACAGTTCTGAGACCG
sequencing_primer_5SQ-5CH6-F GCCGCTGCTTCCTGTGAC
vector_types
Mammalian Expression
growth strain :
Soluble human PACE/furin protease expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI/NheI
cloning_site_5AbsI
promoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha 1)
sequencing_primer_3SQ-3CH1-R ACAAACAGTTCTGAGACCG
sequencing_primer_5SQ-5CH6-F GCCGCTGCTTCCTGTGAC
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesFUR, PACE, PCSK3, SPC1
geneFURIN
id5045
genbank_ids
NM_002569
mutation
nameHuman PACE/furin protease soluble
shRNA_sequence
size2166
species
9606
Homo sapiens
tags
plasmid copy :
Soluble human PACE/furin ORF was obtained by PCR using plasmid SC118550 (Origene, USA) as a template, the ORF was brought in line with the reference sequence NM_002569 by adding six missing nucleotides (coding VQ).
resistance markers :
5212
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA