ST-2xTstem(5A)-GFP | Addgene 162504 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

ST-2xTstem(5A)-GFP

catalog :

162504

citations: 1

Reference
Sun X, Tie H, Chen B, Lu L. Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. J Biol Chem. 2020;295:14750-14762 pubmed publisher

product information

Catalog Number :

162504

Product Name :

ST-2xTstem(5A)-GFP

article :

doi	10.1074/jbc.RA120.014476
id	28215459
pubmed_id	32826314

bacterial resistance :

Kanamycin

cloning :

backbone

pmoxGFP-N1

backbone_mutation

backbone_origin

A gift from E. Snapp (Addgene plasmid # 68070)

backbone_size

3959

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	XhoI
promoter
sequencing_primer_3	SV40-PArev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDw75, SIAT1, ST6GalI, ST6N
gene	ST6GAL1
id	6480

aliases	CD25, IDDM10, IL2R, IMD41, TCGFR, p55
gene	IL2RA
id	3559

genbank_ids

BC040009.1

NM_000417.3

mutation

One Tac stem region is inserted within ST6Gal1 and the other Tac stem region with mutations is inserted between ST6Gal1 and GFP.

name

GFP tagged ST6Gal1 mutant

shRNA_sequence

size

2127

species

9606

Homo sapiens