This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-CD8a(7A)
catalog :
162498
citations: 1
Reference
Sun X, Tie H, Chen B, Lu L. Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. J Biol Chem. 2020;295:14750-14762 pubmed publisher
product information
Catalog Number :
162498
Product Name :
GFP-CD8a(7A)
article :
doi10.1074/jbc.RA120.014476
id28215459
pubmed_id32826314
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutationThe signal peptide of Tac was inserted before GFP tag
backbone_originClontech
backbone_size4770
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI
promoter
sequencing_primer_3SV40-PArev
sequencing_primer_5EGFP-CF
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD8, CD8alpha, Leu2, p32
geneCD8A
id925
genbank_ids
NM_001145873.1
mutationThe five threonine and two serine residues are mutated into alanine residues in the stem region of CD8a
nameCD8a with a mutated stem region
shRNA_sequence
size642
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
resistance markers :
3183
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA