GFP-Cstem-Tac(5A) | Addgene 162497 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

GFP-Cstem-Tac(5A)

catalog :

162497

citations: 1

Reference
Sun X, Tie H, Chen B, Lu L. Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. J Biol Chem. 2020;295:14750-14762 pubmed publisher

product information

Catalog Number :

162497

Product Name :

GFP-Cstem-Tac(5A)

article :

doi	10.1074/jbc.RA120.014476
id	28215459
pubmed_id	32826314

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

The signal peptide of Tac was inserted before GFP tag

backbone_origin

Clontech

backbone_size

4770

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).

origin :

pi :

alt_names

IL2 receptor alpha subunit

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	XhoI
promoter
sequencing_primer_3	SV40-PArev
sequencing_primer_5	EGFP-CF
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD25, IDDM10, IL2R, IMD41, TCGFR, p55
gene	IL2RA
id	3559

aliases	CD8, CD8alpha, Leu2, p32
gene	CD8A
id	925

genbank_ids

NM_000417.3

NM_001145873.1

mutation

The five threonine residues are mutated into alanine residues in the stem region of Tac

name

CD8a stem region and Tac full length with mutated stem region

shRNA_sequence

size

885

species

9606

Homo sapiens