This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Tac(5A)-GFP
catalog :
162495
citations: 1
Reference
Sun X, Tie H, Chen B, Lu L. Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. J Biol Chem. 2020;295:14750-14762 pubmed publisher
product information
Catalog Number :
162495
Product Name :
Tac(5A)-GFP
article :
doi10.1074/jbc.RA120.014476
id28215459
pubmed_id32826314
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4701
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
origin :
37
pi :
alt_names
IL2 receptor alpha subunit
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NheI
promoter
sequencing_primer_3EGFP-NR
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD25, IDDM10, IL2R, IMD41, TCGFR, p55
geneIL2RA
id3559
genbank_ids
NM_000417.3
mutationThe five threonine residues are mutated into alanine residues in the stem region
nameTac with five threonine residues mutated into alanine residues in the stem region
shRNA_sequence
size822
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagGFP
resistance markers :
3183
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA