This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-Tac-STC(5A)
catalog :
162491
citations: 1
Reference
Sun X, Tie H, Chen B, Lu L. Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. J Biol Chem. 2020;295:14750-14762 pubmed publisher
product information
Catalog Number :
162491
Product Name :
GFP-Tac-STC(5A)
article :
doi10.1074/jbc.RA120.014476
id28215459
pubmed_id32826314
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutationThe signal peptide of Tac was inserted before GFP tag
backbone_originClontech
backbone_size4770
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
origin :
37
pi :
alt_names
IL2 receptor alpha subunit
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI
promoter
sequencing_primer_3SV40-PArev
sequencing_primer_5EGFP-CF
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD25, IDDM10, IL2R, IMD41, TCGFR, p55
geneIL2RA
id3559
genbank_ids
NM_000417.3
mutationTac is in partial length with its stem region, TMD and cysotolic tail. The five threonine residues in stem region are mutated into alanine residues.
namepartial length Tac (stem region with mutations, TMD and cytosolic tail)
shRNA_sequence
size177
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
resistance markers :
3183
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA