This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Flag p21 WT
catalog :
16240
citations: 16
Reference
Katuwal N, Kang M, Ghosh M, Hong S, Jeong Y, Park S, et al. Targeting PEG10 as a novel therapeutic approach to overcome CDK4/6 inhibitor resistance in breast cancer. J Exp Clin Cancer Res. 2023;42:325 pubmed publisher
Schultz C, Zhang Y, Elmeskini R, Zimmermann A, Fu H, Murai Y, et al. ATR inhibition augments the efficacy of lurbinectedin in small-cell lung cancer. EMBO Mol Med. 2023;15:e17313 pubmed publisher
Cullot G, Boutin J, Fayet S, Prat F, Rosier J, Cappellen D, et al. Cell cycle arrest and p53 prevent ON-target megabase-scale rearrangements induced by CRISPR-Cas9. Nat Commun. 2023;14:4072 pubmed publisher
Brauer E, Lange T, Keller D, G xf6 rlitz S, Cho S, Keye J, et al. Dissecting the influence of cellular senescence on cell mechanics and extracellular matrix formation in vitro. Aging Cell. 2022;:e13744 pubmed publisher
Chen P, Hussmann J, Yan J, Knipping F, Ravisankar P, Chen P, et al. Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell. 2021;184:5635-5652.e29 pubmed publisher
Kim H, Kang M, Kim M, Shin Y, Chi S, Jeong J. Transcriptional Repression of High-Mobility Group Box 2 by p21 in Radiation-Induced Senescence. Mol Cells. 2018;41:362-372 pubmed publisher
Louis Sam Titus A, Yusuff T, Cassar M, Thomas E, Kretzschmar D, D Mello S. Reduced Expression of Foxp1 as a Contributing Factor in Huntington's Disease. J Neurosci. 2017;37:6575-6587 pubmed publisher
Nagano T, Nakano M, Nakashima A, Onishi K, Yamao S, Enari M, et al. Identification of cellular senescence-specific genes by comparative transcriptomics. Sci Rep. 2016;6:31758 pubmed publisher
Badia R, Pujantell M, Riveira Munoz E, Puig T, Torres Torronteras J, Marti R, et al. The G1/S Specific Cyclin D2 Is a Regulator of HIV-1 Restriction in Non-proliferating Cells. PLoS Pathog. 2016;12:e1005829 pubmed publisher
Pfister J, D Mello S. Regulation of Neuronal Survival by Nucleophosmin 1 (NPM1) Is Dependent on Its Expression Level, Subcellular Localization, and Oligomerization Status. J Biol Chem. 2016;291:20787-97 pubmed publisher
Llanos S, García Pedrero J, Morgado Palacin L, Rodrigo J, Serrano M. Stabilization of p21 by mTORC1/4E-BP1 predicts clinical outcome of head and neck cancers. Nat Commun. 2016;7:10438 pubmed publisher
Ho D, Kim H, Kim J, Sim H, Ahn H, Kim J, et al. Leucine-Rich Repeat Kinase 2 (LRRK2) phosphorylates p53 and induces p21(WAF1/CIP1) expression. Mol Brain. 2015;8:54 pubmed publisher
Neise D, Sohn D, Stefanski A, Goto H, Inagaki M, Wesselborg S, et al. The p90 ribosomal S6 kinase (RSK) inhibitor BI-D1870 prevents gamma irradiation-induced apoptosis and mediates senescence via RSK- and p53-independent accumulation of p21WAF1/CIP1. Cell Death Dis. 2013;4:e859 pubmed publisher
Dai M, Al Odaini A, Fils Aimé N, Villatoro M, Guo J, Arakelian A, et al. Cyclin D1 cooperates with p21 to regulate TGF?-mediated breast cancer cell migration and tumor local invasion. Breast Cancer Res. 2013;15:R49 pubmed publisher
Dai M, Al Odaini A, Arakelian A, Rabbani S, Ali S, Lebrun J. A novel function for p21Cip1 and acetyltransferase p/CAF as critical transcriptional regulators of TGF?-mediated breast cancer cell migration and invasion. Breast Cancer Res. 2012;14:R127 pubmed publisher
Zhou B, Liao Y, Xia W, Spohn B, Lee M, Hung M. Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells. Nat Cell Biol. 2001;3:245-52 pubmed
product information
Catalog Number :
16240
Product Name :
Flag p21 WT
article :
doi10.1038/35060032
id1767
pubmed_id11231573
bacterial resistance :
Ampicillin
cloning :
backbonepMT5
backbone_mutation
backbone_origin
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Order of elements: CMV promoter--Flag--NdeI--ATG--human p21--TGA--BamHI.
origin :
37
pi :
alt_names
Cip1/WAF1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
geneCDKN1A
id1026
genbank_ids
mutation
namep21
shRNA_sequence
size600
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
resistance markers :
215
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA