This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTet-O-ATOH1-T2A-PuroR
catalog :
162342
citations: 1
Reference
Black J, McCutcheon S, Dube S, Barrera A, Klann T, Rice G, et al. Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens. Cell Rep. 2020;33:108460 pubmed publisher
product information
Catalog Number :
162342
Product Name :
pTet-O-ATOH1-T2A-PuroR
article :
doi10.1016/j.celrep.2020.108460
id28215460
pubmed_id33264623
bacterial resistance :
Ampicillin
cloning :
backboneTet-O-FUW
backbone_mutation
backbone_origin
backbone_size8000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral expression of ATOH1 under the control of the TetON promoter
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterTet-ON
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATH1, DFNA89, HATH1, MATH-1, bHLHa14
geneATOH1
id474
genbank_ids
mutation
nameATOH1
shRNA_sequence
size1062
species
9606
Homo sapiens
tags
locationC terminal on insert
tagT2A-PuroR
plasmid copy :
This plasmid was modifed from a gift from Marius Wernig (Addgene plasmid # 52047)
resistance markers :
1264
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA