This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pICE-EGFP-FLAG-PSMD2
catalog :
161917
citations: 1
Reference
Demange P, Joly E, Marcoux J, Zanon P, Listunov D, Rulliere P, et al. SDR enzymes oxidize specific lipidic alkynylcarbinols into cytotoxic protein-reactive species. elife. 2022;11: pubmed publisher
product information
Catalog Number :
161917
Product Name :
pICE-EGFP-FLAG-PSMD2
article :
doi10.7554/eLife.73913
id28215407
pubmed_id35535493
bacterial resistance :
Ampicillin
cloning :
backbonepICE-EGFP-FLAG+(Ku70siR-WT)
backbone_mutationReplaced Ku70 by PSMD2
backbone_originAddgene #46961
backbone_size5743
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3SEQ-BGH-R (TAGAAGGCACAGTCGAGG)
sequencing_primer_5SEQ-GFPC-F (CATGGTCCTGCTGGAGTTCGTG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesP97, RPN1, S2, TRAP2
genePSMD2
id5708
genbank_ids
mutation
name26S proteasome non-ATPase regulatory subunit 2
shRNA_sequence
size2727
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP-FLAG
resistance markers :
5194
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA