pENTR-PKCG | Addgene 16180 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pENTR-PKCG

catalog :

16180

citations: 2

Reference
Stephens D, Powell T, Taraska J, Harris D. Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells. Lipids Health Dis. 2020;19:195 pubmed publisher
Lim J, Hao T, Shaw C, Patel A, Szabo G, Rual J, et al. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell. 2006;125:801-14 pubmed

product information

Catalog Number :

16180

Product Name :

pENTR-PKCG

article :

doi	10.1016/j.cell.2006.03.032
id	1737
pubmed_id	16713569

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

backbone_size

5005

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 Donor vector using the Gateway system as described (Rual et al., 2004), resulting in Entry clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGCTGGTCTGGGCCCC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG CATGACGGGCACAGGCACT-3'.

origin :

pi :

alt_names

PRKCG

cloning

clone_method	Gateway Cloning
cloning_site_3	attP2
cloning_site_5	attP1
promoter
sequencing_primer_3	M13R
sequencing_primer_5	M13F
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	PKC-gamma, PKCC, PKCG, PKCI(3), PKCgamma, SCA14
gene	PRKCG
id	5582

genbank_ids

mutation

name

PRKC gamma

shRNA_sequence

size

2091

species

9606

Homo sapiens