This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-hACE2-hygro
catalog :
161758
citations: 5
Reference
Seixas M, Bartolomeo C, Lemes R, Nicoliche T, Okuda L, Martins L, et al. Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection. Biomater Biosyst. 2023;11:100082 pubmed publisher
Xu D, Jiang W, Wu L, Gaudet R, Park E, Su M, et al. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature. 2023;619:819-827 pubmed publisher
Song N, Deng L, Zeng L, He L, Liu C, Liu L, et al. USP9X deubiquitinates and stabilizes CDC123 to promote breast carcinogenesis through regulating cell cycle. Mol Carcinog. 2023;62:1487-1503 pubmed publisher
Daniloski Z, Jordan T, Ilmain J, Guo X, Bhabha G, tenOever B, et al. The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types. elife. 2021;10: pubmed publisher
Daniloski Z, Jordan T, Wessels H, Hoagland D, Kasela S, Legut M, et al. Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells. Cell. 2021;184:92-105.e16 pubmed publisher
product information
Catalog Number :
161758
Product Name :
pLenti-hACE2-hygro
article :
doi10.1016/j.cell.2020.10.030
id28215374
pubmed_id33147445
bacterial resistance :
Ampicillin
cloning :
backbonepLentiEGFPdestablized
backbone_mutationEGFP-PEST sequence was removed
backbone_origin
backbone_size8770
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses human ACE2 in mammalian cells
origin :
37
pi :
alt_names
hACE2, ACE2
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3caaggaggagaaaatgaaagcc
sequencing_primer_5ggcacctcgattagttctcgag
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACEH
geneACE2
id59272
genbank_ids
mutation
nameHuman ACE2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagP2A
resistance markers :
4810
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA