This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pENTR-ATXN1L
catalog :
16167
citations: 1
Reference
Lim J, Hao T, Shaw C, Patel A, Szabo G, Rual J, et al. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell. 2006;125:801-14 pubmed
product information
Catalog Number :
16167
Product Name :
pENTR-ATXN1L
article :
doi10.1016/j.cell.2006.03.032
id1737
pubmed_id16713569
bacterial resistance :
Spectinomycin
cloning :
backbonepDONR223
backbone_mutation
backbone_origin
backbone_size5005
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 Donor vector using the Gateway system as described (Rual et al., 2004), resulting in Entry clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGGGAGAACTACCAGCTG-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG TTTTCCCGCATTGGAACGCC-3'.
origin :
37
pi :
alt_names
ATXN1L
cloning
clone_methodGateway Cloning
cloning_site_3attP2
cloning_site_5attP1
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesBOAT, BOAT1
geneATXN1L
id342371
genbank_ids
mutation
nameSimilar to Ataxin1
shRNA_sequence
size1992
species
9606
Homo sapiens
tags
resistance markers :
617
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA