pET20b-delta133HtrA2 G399S | Addgene 16158 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET20b-delta133HtrA2 G399S

catalog :

16158

citations: 2

Reference
Merski M, Moreira C, Abreu R, Ramos M, Fernandes P, Martins L, et al. Molecular motion regulates the activity of the Mitochondrial Serine Protease HtrA2. Cell Death Dis. 2017;8:e3119 pubmed publisher
Strauss K, Martins L, Plun Favreau H, Marx F, Kautzmann S, Berg D, et al. Loss of function mutations in the gene encoding Omi/HtrA2 in Parkinson's disease. Hum Mol Genet. 2005;14:2099-111 pubmed

product information

Catalog Number :

16158

Product Name :

pET20b-delta133HtrA2 G399S

article :

doi	10.1093/hmg/ddi215
id	1254
pubmed_id	15961413

bacterial resistance :

Ampicillin

cloning :

backbone

pET20b

backbone_mutation

backbone_origin

Novagen

backbone_size

3584

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

This construct was used in experiments described in figure 4

growth temp :

When transformed and plated on LB/agar/Amp plates, this plasmid will normally only produce colonies if the plates are incubated at room temp as opposed to 37oC. Following this, the bacteria when inoculated in liquid media will grow well at 37oC.

origin :

pi :

alt_names

Omi

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NdeI
promoter	T7
sequencing_primer_3	T7term
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MGCA8, OMI, PARK13, PRSS25
gene	HTRA2
id	27429

genbank_ids

NM_013247

mutation

G399S

name

HtrA2

shRNA_sequence

size

978

species

9606

Homo sapiens