pBAD/myc-his A Rv0712 (FGE) | Addgene 16132 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBAD/myc-his A Rv0712 (FGE)

catalog :

16132

citations: 9

Reference
Asiimwe N, Al Mazid M, Jeong Y, Lee J, Lee J. The discovery of penta-peptides inhibiting the activity of the formylglycine-generating enzyme and their potential antibacterial effects against Mycobacterium tuberculosis. RSC Adv. 2022;12:18884-18888 pubmed publisher
Jiang C, Jiang H, Zhang T, Lu Z, Mao X. Enzymatic Verification and Comparative Analysis of Carrageenan Metabolism Pathways in Marine Bacterium Flavobacterium algicola. Appl Environ Microbiol. 2022;88:e0025622 pubmed publisher
Kim S, Loeff L, Colombo S, Jergic S, Brouns S, Joo C. Selective loading and processing of prespacers for precise CRISPR adaptation. Nature. 2020;579:141-145 pubmed publisher
Hettle A, Hobbs J, Pluvinage B, Vickers C, Abe K, Salama Alber O, et al. Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species. Commun Biol. 2019;2:474 pubmed publisher
Hettle A, Vickers C, Robb C, Liu F, Withers S, Hehemann J, et al. The Molecular Basis of Polysaccharide Sulfatase Activity and a Nomenclature for Catalytic Subsites in this Class of Enzyme. Structure. 2018;26:747-758.e4 pubmed publisher
Bain F, Fischer L, Chen R, Wold M. Single-Molecule Analysis of Replication Protein A-DNA Interactions. Methods Enzymol. 2018;600:439-461 pubmed publisher
Zang B, Ren J, Xu L, Jia L. Direct site-specific immobilization of protein A via aldehyde-hydrazide conjugation. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1008:132-138 pubmed publisher
Shi X, Jung Y, Lin L, Liu C, Wu C, Cann I, et al. Quantitative fluorescence labeling of aldehyde-tagged proteins for single-molecule imaging. Nat Methods. 2012;9:499-503 pubmed publisher
Carrico I, Carlson B, Bertozzi C. Introducing genetically encoded aldehydes into proteins. Nat Chem Biol. 2007;3:321-2 pubmed

product information

Catalog Number :

16132

Product Name :

pBAD/myc-his A Rv0712 (FGE)

article :

doi	10.1038/nchembio878
id	1735
pubmed_id	17450134

bacterial resistance :

Ampicillin

cloning :

backbone

pBAD/myc-his A

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4081

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The gene encoding Mycobacterium tuberculosis FGE (Rv0712, encoding residues 2-299, GenBank accession NP_215226) was amplified from M. tuberculosis H37Rv genomic DNA and ligated into pBAD/myc-his A between the NcoI and XhoI restriction sites. The FGE gene was placed in frame with the start codon using QuikChange mutagenesis. Please note that this information page previously listed the NcoI cloning site as not destroyed. The site was destroyed during cloning, however, and the page was updated on 6/16/08.

growth strain :

Bacterial expression of Mycobacterium tuberculosis FGE

origin :

pi :

alt_names

m tuberculosis FGE

mtb FGE

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NcoI
promoter
sequencing_primer_3
sequencing_primer_5	pBAD-F
site_3_destroyed
site_5_destroyed	1

entrez_gene

genbank_ids

NP_215226

mutation

name

Formylglycine-generating enzyme

shRNA_sequence

size

903

species

Mycobacterium tuberculosis