This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
modified pcAT7-Glo1
catalog :
160996
citations: 1
Reference
Adamson S, Zhan L, Graveley B. Vex-seq: high-throughput identification of the impact of genetic variation on pre-mRNA splicing efficiency. Genome Biol. 2018;19:71 pubmed publisher
product information
Catalog Number :
160996
Product Name :
modified pcAT7-Glo1
article :
doi10.1186/s13059-018-1437-x
id28215329
pubmed_id29859120
bacterial resistance :
Ampicillin
cloning :
backbonepCAGGS
backbone_mutation
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
growth notes :
Cloning for initial Vex-seq inserts is between the PstI and XbaI sites. The second step for generating the final splicing reporter involves PCR amplifying intron 2 and exon 3 from modified pcAT7-Glo1 using GTGTGGAAGTCTCAGGATCG and AACGGGCCCTCTAGAGC and digesting with XbaI and MfeI.
growth strain :
Splicing reporter backbone
origin :
37
pi :
alt_names
HBB
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCAG
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD113t-C, ECYT6, beta-globin
geneHBB
id3043
genbank_ids
NM_000518
mutationRepeated intron 1 with 40 nucelotide deletion
namehemoglobin subunit beta
shRNA_sequence
size1600
species
9606
Homo sapiens
tags
plasmid copy :
The original version of this plasmid was received from Kristen Lynch at University of Pennsylvania. A 40 bp deletion in intron 1 was made in intron 1 compared to the original pcAT7-Glo1.
resistance markers :
5166
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA