This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MDH1-PGK-GFP microRNA-10b
catalog :
16070
citations: 3
Reference
Duru N, Gernapudi R, Lo P, Yao Y, Wolfson B, Zhang Y, et al. Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells. Oncotarget. 2016;7:47511-47525 pubmed publisher
Ouyang H, Gore J, Deitz S, Korc M. microRNA-10b enhances pancreatic cancer cell invasion by suppressing TIP30 expression and promoting EGF and TGF-? actions. Oncogene. 2014;33:4664-74 pubmed publisher
Ma L, Teruya Feldstein J, Weinberg R. Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 2007;449:682-8 pubmed
product information
Catalog Number :
16070
Product Name :
MDH1-PGK-GFP microRNA-10b
article :
doi10.1038/nature06174
id1714
pubmed_id17898713
bacterial resistance :
Ampicillin
cloning :
backboneMDH1-PGK-GFP 2.0
backbone_mutation
backbone_origin
backbone_size6963
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
The miRNA gene was PCR-amplified from normal human genomic DNA and cloned into the MDH1-PGK-GFP 2.0 retroviral vector. It includes miR-10b stem-loop and ~100bp flanking sequences on both sides.
origin :
37
pi :
alt_names
miR-10b
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3ggatcccaatatttgcatgtcgc
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN10B, hsa-mir-10b, miRNA10B, mir-10b
geneMIR10B
id406903
genbank_ids
mutation
namemicroRNA-10b
shRNA_sequence
size315
species
9606
Homo sapiens
tags
resistance markers :
568
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA