This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGS_128
catalog :
160651
citations: 1
Reference
Sienski G, Narayan P, Bonner J, Kory N, Boland S, Arczewska A, et al. APOE4 disrupts intracellular lipid homeostasis in human iPSC-derived glia. Sci Transl Med. 2021;13: pubmed publisher
product information
Catalog Number :
160651
Product Name :
pGS_128
article :
doi10.1126/scitranslmed.aaz4564
id28215255
pubmed_id33658354
bacterial resistance :
Ampicillin
cloning :
backbonepNH605
backbone_mutationGAL1 promoter replaced with pZ estradiol-inducible promoter
backbone_origin
backbone_size7913
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter
origin :
37
pi :
alt_names
APOE3
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterpZ estradiol inducible promoter
sequencing_primer_3atttagctatttgcttagagctcc
sequencing_primer_5AATATACCTCTATACTTTAACGTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAD2, APO-E, ApoE4, LDLCQ5, LPG
geneAPOE
id348
genbank_ids
mutatione3 allele
nameApolipoprotein E e3
shRNA_sequence
size1023
species
9606
Homo sapiens
tags
location
tagKar2 signal sequence
resistance markers :
293
533
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA