This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-CMV-MARCer-Blast
catalog :
160550
citations: 1
Reference
Vermeer J, Ient J, Markelc B, Kaeppler J, Barbeau L, Groot A, et al. A lineage-tracing tool to map the fate of hypoxic tumour cells. Dis Model Mech. 2020;: pubmed publisher
product information
Catalog Number :
160550
Product Name :
pLenti-CMV-MARCer-Blast
article :
doi10.1242/dmm.044768
id28214792
pubmed_id32571767
bacterial resistance :
Ampicillin
cloning :
backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
backbone_mutation
backbone_origin
backbone_size7592
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
labeling hypoxic cells
origin :
30
pi :
alt_names
insert encodes amino acids 1-603 of the human HIF-1 protein fused to eGFP-CreERT2
cloning
clone_methodRestriction Enzyme
cloning_site_3BstXI
cloning_site_5BstXI
promoterCMV
sequencing_primer_3WPRE-R
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
geneHIF1A
id3091
genbank_ids
mutation
nameMARCer
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tageGFP-Cre
resistance markers :
5145
tags :
Low Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA