This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1/Myc-His beta4
catalog :
16039
citations: 6
Reference
Zhang W, Lu C, Nakamoto M, Tsai C, Roy A, Lee C, et al. Curved adhesions mediate cell attachment to soft matrix fibres in three dimensions. Nat Cell Biol. 2023;25:1453-1464 pubmed publisher
Zhang W, Lu C, Nakamoto M, Tsai C, Roy A, Lee C, et al. Curved adhesions mediate cell attachment to soft matrix fibres in 3D. bioRxiv. 2023;: pubmed publisher
Qi L, Knifley T, Chen M, O Connor K. Integrin α6β4 requires plectin and vimentin for adhesion complex distribution and invasive growth. J Cell Sci. 2022;135: pubmed publisher
Myllymäki S, Kämäräinen U, Liu X, Cruz S, Miettinen S, Vuorela M, et al. Assembly of the β4-Integrin Interactome Based on Proximal Biotinylation in the Presence and Absence of Heterodimerization. Mol Cell Proteomics. 2019;18:277-293 pubmed publisher
Brown C, Amante J, Goel H, Mercurio A. The α6β4 integrin promotes resistance to ferroptosis. J Cell Biol. 2017;216:4287-4297 pubmed publisher
Dans M, Gagnoux Palacios L, Blaikie P, Klein S, Mariotti A, Giancotti F. Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of hemidesmosomes. J Biol Chem. 2001;276:1494-502 pubmed
product information
Catalog Number :
16039
Product Name :
pcDNA3.1/Myc-His beta4
article :
doi10.1074/jbc.M008663200
id1705
pubmed_id11044453
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(-)/Myc-His B
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note: The plasmid contains a S1694T mutation compared to NCBI reference sequence AAC51632.1. This mutation is a natural variant which is not expected to affect plasmid function.
origin :
37
pi :
alt_names
beta 4 integrin
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD104, GP150, JEB5A, JEB5B
geneITGB4
id3691
genbank_ids
mutation
namebeta4
shRNA_sequence
size5600
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagMyc
locationC terminal on backbone
tagHis
resistance markers :
165
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA