This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGL3-FLIP1500
catalog :
16016
citations: 3
Reference
Booth L, West C, Von Hoff D, Kirkwood J, Dent P. GZ17-6.02 Interacts With [MEK1/2 and B-RAF Inhibitors] to Kill Melanoma Cells. Front Oncol. 2021;11:656453 pubmed publisher
Hwang I, Oh S, Jang H, Jo E, Joo J, Lee K, et al. Cordycepin promotes apoptosis in renal carcinoma cells by activating the MKK7-JNK signaling pathway through inhibition of c-FLIPL expression. PLoS ONE. 2017;12:e0186489 pubmed publisher
Ricci M, Jin Z, Dews M, Yu D, Thomas Tikhonenko A, Dicker D, et al. Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity. Mol Cell Biol. 2004;24:8541-55 pubmed
product information
Catalog Number :
16016
Product Name :
pGL3-FLIP1500
article :
doi10.1128/MCB.24.19.8541-8555.2004
id1690
pubmed_id15367674
bacterial resistance :
Ampicillin
cloning :
backbonepGL3-basic
backbone_mutation
backbone_originPromega
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
A 1,460-bp sequence containing the promoter region of the FLIP gene was amplified using the BAC clone RP11-536118 (Children s Hospital Oakland Research Institute, Oakland, Calif.) and inserted into the luciferase-expressing reporter plasmid pGL3-basic (Promega). The sequence corresponds to positions 1179 to +281 relative to the proposed transcriptional start site of CFLAR (FLIP) exon 1 (accession no. AB038965).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3LucNrev
sequencing_primer_5RVprimer3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP
geneCFLAR
id8837
genbank_ids
AB038965
mutation
nameFLIP
shRNA_sequence
size1460
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagLuciferase
resistance markers :
129
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA