This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p405TEF1
catalog :
15968
citations: 1
Reference
Lilley A, Major L, Young S, Stark M, Smith T. The essential roles of cytidine diphosphate-diacylglycerol synthase in bloodstream form Trypanosoma brucei. Mol Microbiol. 2014;92:453-70 pubmed publisher
product information
Catalog Number :
15968
Product Name :
p405TEF1
article :
doi
id1673
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonep405GALS
backbone_mutation
backbone_originN. Buchler
backbone_size5074
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Yeast Expression
origin :
37
pi :
alt_names
Translational elongation factor EF-1 alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3NgoMIV
cloning_site_5SacI
promoter
sequencing_primer_3
sequencing_primer_5M13 Reverse
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPR080W
geneTEF1
id856195
genbank_ids
mutationTEF1 promoter is between SacI and XbaI restriction sites.
nameTEF1 promoter + polylinker + CYC1 terminator
shRNA_sequence
size1060
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
Insert was cut from p404TEF1, which was obtained from Ben Timney.
resistance markers :
55
99
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA