This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTYF-PRSx8-AstR-p2A-GFP
catalog :
159632
citations: 1
Reference
Ngo H, Melo M, Layfield S, Connelly A, Bassi J, Xie L, et al. A Chemogenetic Tool that Enables Functional Neural Circuit Analysis. Cell Rep. 2020;32:108139 pubmed publisher
product information
Catalog Number :
159632
Product Name :
pTYF-PRSx8-AstR-p2A-GFP
article :
doi10.1016/j.celrep.2020.108139
id28211968
pubmed_id32937120
bacterial resistance :
Ampicillin
cloning :
backbonepTYF
backbone_mutation
backbone_origin
backbone_size7835
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Plasmid features: AstR: 338 -1521, P2A: 1529 -1597, GFP: 1598 -2311 ,WPRE: 2373- 2969 Please note: Plasmid contains a 16bp deletion at the start of L-ITR. This deletion has no functional effect on the plasmid.
growth strain :
Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter
origin :
37
pi :
alt_names
Allatostatin receptor, AstR, AlstR,
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5SpeI
promoterPRSx8
sequencing_primer_3GFP F: CAAAGACCCCAACGAGA
sequencing_primer_5PRSx8 F: CAGGTGGGACCAGAGAGCTCACCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG2872, ASR, AST-R, AlstR, AstA R1, AstAR1, CG2872, D.AlstR1, DAR-1, DAR1, DGR, Dar-1, Dmel CG2872, EG:121E7.2, GR
geneAstA-R1
id44126
genbank_ids
AF163775.1
mutation
nameAstR
shRNA_sequence
size1184
species
7227
Drosophila melanogaster
tags
locationC terminal on insert
tagP2A
locationC terminal on insert
tagGFP
plasmid copy :
AstR sequence was cloned from addgene plasmid #14895
resistance markers :
5105
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA