pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE | Addgene 159630 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

catalog :

159630

citations: 1

Reference
Ngo H, Melo M, Layfield S, Connelly A, Bassi J, Xie L, et al. A Chemogenetic Tool that Enables Functional Neural Circuit Analysis. Cell Rep. 2020;32:108139 pubmed publisher

product information

Catalog Number :

159630

Product Name :

pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

article :

doi	10.1016/j.celrep.2020.108139
id	28211968
pubmed_id	32937120

bacterial resistance :

Ampicillin

cloning :

backbone

pAM

backbone_mutation

backbone_origin

backbone_size

5364

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

growth notes :

Plasmid features: CAG promoter: 271 -1152, Allatostatin: 1159- 1563, IRES: 1584- 2124, mCherry: 2155- 2859, WPRE: 2881- 3468 Please note: Plasmid contains K4M and G5D mutations in mCherry. These mutations do not affect plasmid function.

growth strain :

AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

origin :

pi :

alt_names

Allatostatin-3, Ast,

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter	DCA (same as CAG promoter)
sequencing_primer_3	mCherry R: TTGGTCACCTTCAGCTTGG
sequencing_primer_5	HA F: TACCCATACGACGTCCCAGA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG13633, ALLS, AS, ASA, AST, AST-1, AST-3, AST-4, AST-A, Allatostatin A1, Allatostatin A2, Allatostatin A3, Allatostatin A4, Ast, Ast-A, AstA-1, AstA-2, AstA-3, AstA-4, AstA1, AstA2, AstA4, BcDNA:RE16553, CG13633, DAP, DAP-A, DST-1A, DST-2A, DST-3A, DST-4A, Dmel CG13633, Drm-AST-1, Drm-AST-2, Drm-AST-3, Drm-AST-4, Drm-AST-A, ast
gene	AstA
id	42947

genbank_ids

AE014297.3

mutation

name

Ast

shRNA_sequence

size

1700

species

7227

Drosophila melanogaster