This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p405MET3
catalog :
15962
product information
Catalog Number :
15962
Product Name :
p405MET3
article :
doi
id1673
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonep405MET3b
backbone_mutation
backbone_originA. Geoghegan (Rockefeller University)
backbone_size5578
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Yeast Expression
origin :
37
pi :
alt_names
ATP sulfurylase
cloning
clone_methodRestriction Enzyme
cloning_site_3NgoMIV
cloning_site_5XbaI
promoter
sequencing_primer_3
sequencing_primer_5M13 Reverse
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneMET3
id853466
genbank_ids
mutationMET3 promoter is between SacI and XbaI restriction sites.
nameMET3 promoter + polylinker + CYC1 terminator
shRNA_sequence
size649
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
Original vector p405MET3b was created by subcloning PCR fragment of genomic MET3 promoter (with SacI and XbaI restriction sites introduced) into SacI-XbaI cut vector pRS405. This plasmid does not contains CYC1 terminator. Plasmid p405MET3 was created by subcloning XbaI-NgoMIV insert from p405GALS (insert = polylinker + CYC1 terminator) into XbaI-NgoMIV cut p405MET3b vector.
resistance markers :
55
99
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA