This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pQE30-VRN1-R249E-R289E-R296E
catalog :
159532
citations: 1
Reference
King G, Chanson A, McCallum E, Ohme Takagi M, Byriel K, Hill J, et al. The Arabidopsis B3 domain protein VERNALIZATION1 (VRN1) is involved in processes essential for development, with structural and mutational studies revealing its DNA-binding surface. J Biol Chem. 2013;288:3198-207 pubmed publisher
product information
Catalog Number :
159532
Product Name :
pQE30-VRN1-R249E-R289E-R296E
article :
doi10.1074/jbc.M112.438572
id28211941
pubmed_id23255593
bacterial resistance :
Ampicillin
cloning :
backbonepQE30
backbone_mutation
backbone_originQiagen
backbone_size3433
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli
origin :
37
pi :
alt_names
VRN1
At3g18990
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoterT5
sequencing_primer_3GTTCTGAGGTCATTACTGG
sequencing_primer_5CCCGAAAAGTGCCACCTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G18990, REDUCED VERNALIZATION RESPONSE 1, REM39, REPRODUCTIVE MERISTEM 39
geneVRN1
id821432
genbank_ids
mutationThree charge reversal mutations, namely R249E R289E R296E
nameVERNALIZATION1
shRNA_sequence
size411
species
3702
Arabidopsis thaliana
tags
locationN terminal on backbone
tagSix-Histidine tag
resistance markers :
4106
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA