This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AAVS1-PDL1
catalog :
159280
citations: 1
Reference
Ma H, Jeppesen J, Jaenisch R. Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction. Cell Rep Med. 2020;1:100097 pubmed publisher
product information
Catalog Number :
159280
Product Name :
AAVS1-PDL1
article :
doi10.1016/j.xcrm.2020.100097
id28211889
pubmed_id33205073
bacterial resistance :
Ampicillin
cloning :
backbonepUC57
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
Other
S1 knockin donor vector
growth strain :
Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3PacI
cloning_site_5AgeI
promoterCAGGS
sequencing_primer_3ttggcagagggaaaaagatctcagtgg
sequencing_primer_5ggcggctctagagcctctgctaacc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1
geneCD274
id29126
genbank_ids
mutation
nameHuman PDL1/CD274
shRNA_sequence
size873
species
9606
Homo sapiens
tags
resistance markers :
227
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA