This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-HA-DEPTOR S286/287/291A
catalog :
159160
citations: 1
Reference
Duan S, Skaar J, Kuchay S, Toschi A, Kanarek N, Ben Neriah Y, et al. mTOR generates an auto-amplification loop by triggering the ?TrCP- and CK1?-dependent degradation of DEPTOR. Mol Cell. 2011;44:317-24 pubmed publisher
product information
Catalog Number :
159160
Product Name :
pcDNA3-HA-DEPTOR S286/287/291A
article :
doi10.1016/j.molcel.2011.09.005
id28211877
pubmed_id22017877
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses DEPTOR S286/287/291A mutant in mammalian cells
origin :
37
pi :
alt_names
DEPDC6
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDEP.6, DEPDC6
geneDEPTOR
id64798
genbank_ids
NM_022783.4
mutationchanged serine 286,287 and 291 to alanine
nameDEPTOR
shRNA_sequence
size1230
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
376
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA