pRK5myc RhoA L63 | Addgene 15900 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRK5myc RhoA L63

catalog :

15900

citations: 7

Reference
Kato T, Ghadban L, Boucher E, Mandato C. Tension modulation of actomyosin ring assembly and RhoGTPases activity: Perspectives from the Xenopus oocyte wound healing model. Cytoskeleton (Hoboken). 2021;78:349-360 pubmed publisher
Bonfim Melo A, Ferreira d, Mortara R. Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi. Front Microbiol. 2018;9:360 pubmed publisher
Jiu Y, Peranen J, Schaible N, Cheng F, Eriksson J, Krishnan R, et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci. 2017;130:892-902 pubmed publisher
Krogh K, Lyddon E, Thayer S. HIV-1 Tat activates a RhoA signaling pathway to reduce NMDA-evoked calcium responses in hippocampal neurons via an actin-dependent mechanism. J Neurochem. 2015;132:354-66 pubmed publisher
Kalia M, Khasa R, Sharma M, Nain M, Vrati S. Japanese encephalitis virus infects neuronal cells through a clathrin-independent endocytic mechanism. J Virol. 2013;87:148-62 pubmed publisher
Pullikuth A, Catling A. Extracellular signal-regulated kinase promotes Rho-dependent focal adhesion formation by suppressing p190A RhoGAP. Mol Cell Biol. 2010;30:3233-48 pubmed publisher
Nobes C, Hall A. Rho GTPases control polarity, protrusion, and adhesion during cell movement. J Cell Biol. 1999;144:1235-44 pubmed

product information

Catalog Number :

15900

Product Name :

pRK5myc RhoA L63

article :

doi	10.1083/jcb.144.6.1235
id	1658
pubmed_id	10087266

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5-myc

backbone_mutation

backbone_origin

backbone_size

4750

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Constitutively activated Rho. There is an additional F25N mutation in the sequence. This change greatly facilitated expression of the protein in E.coli. The only known effect of this mutation may be negatively affect the transduction of RhoA signaling to DGK1 kinase. Otherwise the depositing lab have never found any discrepancies with other published work on activity or protein interactions.

origin :

pi :

alt_names

A isoform of Rho

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARH12, ARHA, EDFAOB, RHO12, RHOH12
gene	RHOA
id	387

genbank_ids

mutation

Q63L

name

RhoA

shRNA_sequence

size

600

species

9606

Homo sapiens