pRK5myc RhoA wt | Addgene 15899 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRK5myc RhoA wt

catalog :

15899

citations: 5

Reference
Guo G, Gong K, Beckley N, Zhang Y, Yang X, Chkheidze R, et al. EGFR ligand shifts the role of EGFR from oncogene to tumour suppressor in EGFR-amplified glioblastoma by suppressing invasion through BIN3 upregulation. Nat Cell Biol. 2022;24:1291-1305 pubmed publisher
Medina C, Ferreira E, Bonifácio B, Mortara R, Bonfim Melo A. Trypanosoma cruzi extracellular amastigotes engage Rac1 and Cdc42 to invade RAW macrophages. Microbes Infect. 2021;:104837 pubmed publisher
Bonfim Melo A, Ferreira d, Mortara R. Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi. Front Microbiol. 2018;9:360 pubmed publisher
Kalia M, Khasa R, Sharma M, Nain M, Vrati S. Japanese encephalitis virus infects neuronal cells through a clathrin-independent endocytic mechanism. J Virol. 2013;87:148-62 pubmed publisher
Nobes C, Hall A. Rho GTPases control polarity, protrusion, and adhesion during cell movement. J Cell Biol. 1999;144:1235-44 pubmed

product information

Catalog Number :

15899

Product Name :

pRK5myc RhoA wt

article :

doi	10.1083/jcb.144.6.1235
id	1658
pubmed_id	10087266

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5-myc

backbone_mutation

backbone_origin

backbone_size

4750

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

There is an additional F25N mutation in the sequence. This change greatly facilitated expression of the protein in E.coli. The only known effect of this mutation may be negatively affect the transduction of RhoA signaling to DGK1 kinase. Otherwise the depositing lab have never found any discrepancies with other published work on activity or protein interactions.

origin :

pi :

alt_names

A isoform of Rho

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARH12, ARHA, EDFAOB, RHO12, RHOH12
gene	RHOA
id	387

genbank_ids

mutation

name

RhoA

shRNA_sequence

size

600

species

9606

Homo sapiens