This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)
catalog :
158953
citations: 1
Reference
Marzio A, Puccini J, Kwon Y, Maverakis N, Arbini A, Sung P, et al. The F-Box Domain-Dependent Activity of EMI1 Regulates PARPi Sensitivity in Triple-Negative Breast Cancers. Mol Cell. 2019;73:224-237.e6 pubmed publisher
product information
Catalog Number :
158953
Product Name :
pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)
article :
doi10.1016/j.molcel.2018.11.003
id28197720
pubmed_id30554948
bacterial resistance :
Ampicillin
cloning :
backbonepcdna3.1
backbone_mutation
backbone_origin
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Express human RAD51(T309A) mutant in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5Bamh1
promoterCMV
sequencing_primer_3BGHR reverse
sequencing_primer_5CMV forward
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesBRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA
geneRAD51
id5888
genbank_ids
49168601
mutationchanged threonine 309 to alanine
nameRAD51
shRNA_sequence
size1020
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag2X FLAG 2X STREP
resistance markers :
376
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA