This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPICZ-His6-K2P4.1B-mCherry-StreptagII
catalog :
158744
citations: 2
Reference
Zhu Y, Yun S, Zhang T, Chang J, Stover L, Laganowsky A. Native mass spectrometry of proteoliposomes containing integral and peripheral membrane proteins. Chem Sci. 2023;14:14243-14255 pubmed publisher
Schrecke S, Zhu Y, McCabe J, Bartz M, Packianathan C, Zhao M, et al. Selective regulation of human TRAAK channels by biologically active phospholipids. Nat Chem Biol. 2021;17:89-95 pubmed publisher
product information
Catalog Number :
158744
Product Name :
pPICZ-His6-K2P4.1B-mCherry-StreptagII
article :
doi10.1038/s41589-020-00659-5
id28211815
pubmed_id
bacterial resistance :
Bleocin (Zeocin)
cloning :
backbonepPICZ
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.
origin :
37
pi :
alt_names
TRAAK
KCNK4
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterAOX1
sequencing_primer_3GCAAATGGCATTCTGACATCC
sequencing_primer_5GACTGGTTCCAATTGACAAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFHEIG, K2p4.1, TRAAK, TRAAK1
geneKCNK4
id50801
genbank_ids
mutationResidues 1-290 and N-linked glycosylation sites removed (N104Q/N108Q)
nameK2P4.1-B
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis6
locationC terminal on insert
tagmCherry with Streptag II
plasmid copy :
Insert was synthesized by IDT
resistance markers :
4394
tags :
Unknown
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA