This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPICZ-His6-K2P4.1A-mCherry-StreptagII
catalog :
158743
citations: 1
Reference
Schrecke S, Zhu Y, McCabe J, Bartz M, Packianathan C, Zhao M, et al. Selective regulation of human TRAAK channels by biologically active phospholipids. Nat Chem Biol. 2021;17:89-95 pubmed publisher
product information
Catalog Number :
158743
Product Name :
pPICZ-His6-K2P4.1A-mCherry-StreptagII
article :
doi10.1038/s41589-020-00659-5
id28211815
pubmed_id
bacterial resistance :
Bleocin (Zeocin)
cloning :
backbonepPICZ
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.
origin :
37
pi :
alt_names
TRAAK
KCNK4
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterAOX1
sequencing_primer_3GCAAATGGCATTCTGACATCC
sequencing_primer_5GACTGGTTCCAATTGACAAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFHEIG, K2p4.1, TRAAK, TRAAK1
geneKCNK4
id50801
genbank_ids
mutationResidues 1-264 with N-linked glycosylation sites mutated from Asn to Qln
nameK2P4.1-A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis6
locationC terminal on insert
tagmCherry with Streptag II
plasmid copy :
Insert was synthesized by IDT
resistance markers :
4394
tags :
Unknown
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA