pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro | Addgene 158639 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

catalog :

158639

citations: 1

Reference
Lopacinski A, Sweatt A, Smolko C, Gray Gaillard E, Borgman C, Shah M, et al. Modeling the complete kinetics of coxsackievirus B3 reveals human determinants of host-cell feedback. Cell Syst. 2021;12:304-323.e13 pubmed publisher

product information

Catalog Number :

158639

Product Name :

pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

article :

doi
id	28211802
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pSLIK hygro

backbone_mutation

backbone_origin

Addgene #25737

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. For pSLIK vectors, it is recommended to use zeocin in addition to ampicillin for bacterial selection.

growth strain :

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

origin :

pi :

alt_names

IPS-1

VISA

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CARDIF, IPS-1, IPS1, VISA
gene	MAVS
id	57506

genbank_ids

mutation

Q148A/Q271A

name

MAVS

shRNA_sequence

size

species

9606

Homo sapiens