This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEN_TT 3xFLAG-MAVS-Gln93,Ala271
catalog :
158632
citations: 1
product information
Catalog Number :
158632
Product Name :
pEN_TT 3xFLAG-MAVS-Gln93,Ala271
article :
| doi | |
| id | 28211802 |
| pubmed_id |
bacterial resistance :
Gentamicin
cloning :
| backbone | pEN_TTmiR2c_3xFLAG | ||
| backbone_mutation | |||
| backbone_origin | Addgene #83274 | ||
| backbone_size | |||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). This plamid has an additional Q271A mutation, and amino acid 93 (glutamate, E) was mutated back to the reference (glutamine, Q). Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q271A and a second mutagenesis to yield the double mutant. The clones displayed consistent off-target mutations at one of two points. Two clones with different off-target mutations were digested and the good pieces ligated together so to yield the final sequence.
growth strain :
Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant
origin :
37
pi :
|
resistance markers :
| 1585 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments