This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEN_TT 3xFLAG-MAVS-Ala148
catalog :
158630
citations: 1
Reference
Lopacinski A, Sweatt A, Smolko C, Gray Gaillard E, Borgman C, Shah M, et al. Modeling the complete kinetics of coxsackievirus B3 reveals human determinants of host-cell feedback. Cell Syst. 2021;12:304-323.e13 pubmed publisher
product information
Catalog Number :
158630
Product Name :
pEN_TT 3xFLAG-MAVS-Ala148
article :
doi
id28211802
pubmed_id
bacterial resistance :
Gentamicin
cloning :
backbonepEN_TTmiR2c_3xFLAG
backbone_mutation
backbone_originAddgene #83274
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway entry vector
growth notes :
The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F. This plasmid has an additional Q148A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) on the pEN_TT 3XFLAG-MAVS plasmid.
growth strain :
Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant
origin :
37
pi :
alt_names
IPS-1
VISA
cloning
clone_methodRestriction Enzyme
cloning_site_3MfeI
cloning_site_5SpeI
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCARDIF, IPS-1, IPS1, VISA
geneMAVS
id57506
genbank_ids
mutationQ148A
nameMAVS
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag3XFLAG
resistance markers :
1585
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA