pGolf-ISH | Addgene 15853 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGolf-ISH

catalog :

15853

citations: 1

Reference
Hirota J, Omura M, Mombaerts P. Differential impact of Lhx2 deficiency on expression of class I and class II odorant receptor genes in mouse. Mol Cell Neurosci. 2007;34:679-88 pubmed

product information

Catalog Number :

15853

Product Name :

pGolf-ISH

article :

doi	10.1016/j.mcn.2007.01.014
id	1649
pubmed_id	17350283

bacterial resistance :

Ampicillin

cloning :

backbone

pGEM-T Easy

backbone_mutation

backbone_origin

promega

backbone_size

3015

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

cloning vector

growth notes :

This plasmid was used to prepare RNA probes for in situ hybridization (ISH) and has not been used for functional studies of Gnal. It is not known whether the F199S mutation may affect function of Gnal. Moreover, be advised that the plasmid does not contain the full coding region of Gnal.

origin :

pi :

alt_names

Golf

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13 reverse, SP6
sequencing_primer_5	M13, T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	2610011C15Rik, 9630020G10Rik, Galphaolf, Gna10, Golf
gene	Gnal
id	14680

genbank_ids

NM_010307

mutation

F199S

name

Guanine Nucleotide Binding Protein, Alpha Stimulating, Olfactory type

shRNA_sequence

size

1130

species

10090

Mus musculus