This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEMS2214
catalog :
158417
citations: 1
product information
Catalog Number :
158417
Product Name :
pEMS2214
article :
| doi | 10.1038/s41434-021-00227-z |
| id | 28211774 |
| pubmed_id | 33531684 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pEMS2131 | |
| backbone_mutation | ||
| backbone_origin | Elizabeth Simpson (Addgene plasmid # 111895) | |
| backbone_size | 4999 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid.
growth strain :
AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.
growth temp :
SURE cells from Agilent, that the colonies are freshly picked, and the you limit the time to grow the culture. We typically transform the plasmid in the afternoon and take out the plate from the 37 degree incubator in the morning, we then pick the colonies and grow in LB for ~ 20 hrs
inserts :
SURE cells
origin :
37
pi :
|
plasmid copy :
DNA synthesized at GenScript
resistance markers :
| 928 |
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments