This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO_Ollas.C.FLAG_NGFR
catalog :
158311
citations: 1
Reference
Wroblewska A, Dhainaut M, Ben Zvi B, Rose S, Park E, Amir E, et al. Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens. Cell. 2018;175:1141-1155.e16 pubmed publisher
product information
Catalog Number :
158311
Product Name :
pLKO_Ollas.C.FLAG_NGFR
article :
doi10.1016/j.cell.2018.09.022
id28208255
pubmed_id30343902
bacterial resistance :
Ampicillin
cloning :
backbonepLKO
backbone_mutation
backbone_origin
backbone_size9000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
CRISPR
growth strain :
Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5Unknown
promoterEF1a
sequencing_primer_3
sequencing_primer_5cctttttgagtttggatcttggttcat
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR
geneNGFR
id4804
genbank_ids
mutationTruncation of the signaling domain
namePro-Code Tagged human dNGFR
shRNA_sequence
size1000
species
9606
Homo sapiens
tags
locationN terminal on insert
tagOllas.C.FLAG
resistance markers :
1628
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA