This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-DN-hCUL5-FLAG
catalog :
15823
citations: 8
Reference
Zhang Z, Perkovic M, Gu Q, Balakrishnan K, Sangwiman A, Haussinger D, et al. HIV-2 Vif and foamy virus Bet antagonize APOBEC3B by different mechanisms. Virology. 2021;554:17-27 pubmed publisher
Fu S, Hu M, Peng Y, Fang H, Hsiao C, Chen T, et al. CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy. Cells. 2020;9: pubmed publisher
Bailly A, Perrin A, Serrano Macia M, Maghames C, Leidecker O, Trauchessec H, et al. The Balance between Mono- and NEDD8-Chains Controlled by NEDP1 upon DNA Damage Is a Regulatory Module of the HSP70 ATPase Activity. Cell Rep. 2019;29:212-224.e8 pubmed publisher
Gu Q, Zhang Z, Gertzen C, Haussinger D, Gohlke H, Munk C. Identification of a Conserved Interface of Human Immunodeficiency Virus Type 1 and Feline Immunodeficiency Virus Vifs with Cullin 5. J Virol. 2018;92: pubmed publisher
Chang T, Wang S, Chen L, Shih Y, Chang L, Liu S, et al. Regulation of the Abundance of Kaposi's Sarcoma-Associated Herpesvirus ORF50 Protein by Oncoprotein MDM2. PLoS Pathog. 2016;12:e1005918 pubmed publisher
Greenwood E, Matheson N, Wals K, van den Boomen D, Antrobus R, Williamson J, et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. elife. 2016;5: pubmed publisher
Chen Y, Peng Y, Hu M, Huang J, Chien Y, Wu J, et al. The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels. Sci Rep. 2015;5:10667 pubmed publisher
Jin J, Ang X, Shirogane T, Wade Harper J. Identification of substrates for F-box proteins. Methods Enzymol. 2005;399:287-309 pubmed
product information
Catalog Number :
15823
Product Name :
pcDNA3-DN-hCUL5-FLAG
article :
doi10.1016/S0076-6879(05)99020-4
id1644
pubmed_id16338364
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
C-terminal truncation of hCul5: amino acids 1-441.
origin :
37
pi :
alt_names
Cullin 5
Cul5
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCUL-5, VACM-1, VACM1
geneCUL5
id8065
genbank_ids
mutationDominant negative: N441
nameCullin5
shRNA_sequence
size2200
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFlag
resistance markers :
191
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA