This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-DN-hCUL2-FLAG
catalog :
15819
citations: 5
Reference
Wang D, Zhang Q, Li F, Wang C, Yang C, Yu H. β-TrCP-mediated ubiquitination and degradation of Dlg5 regulates hepatocellular carcinoma cell proliferation. Cancer Cell Int. 2019;19:298 pubmed publisher
Gu Q, Zhang Z, Gertzen C, Haussinger D, Gohlke H, Munk C. Identification of a Conserved Interface of Human Immunodeficiency Virus Type 1 and Feline Immunodeficiency Virus Vifs with Cullin 5. J Virol. 2018;92: pubmed publisher
Chang T, Wang S, Chen L, Shih Y, Chang L, Liu S, et al. Regulation of the Abundance of Kaposi's Sarcoma-Associated Herpesvirus ORF50 Protein by Oncoprotein MDM2. PLoS Pathog. 2016;12:e1005918 pubmed publisher
Mudhasani R, Tran J, Retterer C, Kota K, Whitehouse C, Bavari S. Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase. PLoS Pathog. 2016;12:e1005437 pubmed publisher
Jin J, Ang X, Shirogane T, Wade Harper J. Identification of substrates for F-box proteins. Methods Enzymol. 2005;399:287-309 pubmed
product information
Catalog Number :
15819
Product Name :
pcDNA3-DN-hCUL2-FLAG
article :
doi10.1016/S0076-6879(05)99020-4
id1644
pubmed_id16338364
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
C-terminal truncation of hCul2: amino acids 1-427.
origin :
37
pi :
alt_names
Cullin 2
Cul2
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCUL2
id8453
genbank_ids
mutationDominant negative: N427
nameCullin2
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFlag
resistance markers :
191
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA