This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-DN-hCUL1-FLAG
catalog :
15818
citations: 11
Reference
Fu S, Hu M, Peng Y, Fang H, Hsiao C, Chen T, et al. CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy. Cells. 2020;9: pubmed publisher
Manzione M, Rombouts J, Steklov M, Pasquali L, SABLINA A, Gelens L, et al. Co-regulation of the antagonistic RepoMan:Aurora-B pair in proliferating cells. Mol Biol Cell. 2020;31:419-438 pubmed publisher
Wang D, Zhang Q, Li F, Wang C, Yang C, Yu H. β-TrCP-mediated ubiquitination and degradation of Dlg5 regulates hepatocellular carcinoma cell proliferation. Cancer Cell Int. 2019;19:298 pubmed publisher
Suzuki T, Okamoto T, Katoh H, Sugiyama Y, Kusakabe S, Tokunaga M, et al. Infection with flaviviruses requires BCLXL for cell survival. PLoS Pathog. 2018;14:e1007299 pubmed publisher
Chang T, Wang S, Chen L, Shih Y, Chang L, Liu S, et al. Regulation of the Abundance of Kaposi's Sarcoma-Associated Herpesvirus ORF50 Protein by Oncoprotein MDM2. PLoS Pathog. 2016;12:e1005918 pubmed publisher
Lutz L, Pace C, Arnold M. Rotavirus NSP1 Associates with Components of the Cullin RING Ligase Family of E3 Ubiquitin Ligases. J Virol. 2016;90:6036-48 pubmed publisher
Mudhasani R, Tran J, Retterer C, Kota K, Whitehouse C, Bavari S. Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase. PLoS Pathog. 2016;12:e1005437 pubmed publisher
Gao J, Yan J, Xu M, Ren S, Xie W. CAR Suppresses Hepatic Gluconeogenesis by Facilitating the Ubiquitination and Degradation of PGC1α. Mol Endocrinol. 2015;29:1558-70 pubmed publisher
Chen Y, Peng Y, Hu M, Huang J, Chien Y, Wu J, et al. The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels. Sci Rep. 2015;5:10667 pubmed publisher
Hughes D, Wood J, Jackson B, Baquero Pérez B, Whitehouse A. NEDDylation is essential for Kaposi's sarcoma-associated herpesvirus latency and lytic reactivation and represents a novel anti-KSHV target. PLoS Pathog. 2015;11:e1004771 pubmed publisher
Jin J, Ang X, Shirogane T, Wade Harper J. Identification of substrates for F-box proteins. Methods Enzymol. 2005;399:287-309 pubmed
product information
Catalog Number :
15818
Product Name :
pcDNA3-DN-hCUL1-FLAG
article :
doi10.1016/S0076-6879(05)99020-4
id1644
pubmed_id16338364
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
C-terminal truncation of hCul1: amino acids 1-452.
origin :
37
pi :
alt_names
Cullin 1
Cul1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCUL1
id8454
genbank_ids
mutationDominant negative: N452
nameCullin1
shRNA_sequence
size1400
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFlag
resistance markers :
191
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA