pMAH-POLY-eRF1(E55D) | Addgene 157925 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMAH-POLY-eRF1(E55D)

catalog :

157925

citations: 1

Reference
Chen Z, Zhang S, Li X, Ai H. A high-performance genetically encoded fluorescent biosensor for imaging physiological peroxynitrite. Cell Chem Biol. 2021;: pubmed publisher

product information

Catalog Number :

157925

Product Name :

pMAH-POLY-eRF1(E55D)

article :

doi	10.1016/j.chembiol.2021.01.013
id	28211697
pubmed_id	33581056

bacterial resistance :

Ampicillin

cloning :

backbone

pMAH

backbone_mutation

backbone_origin

backbone_size

7583

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This plasmid can be used in mammalian cells to genetically encode an array of para-substituted phenylalanine analogs, including p-boronophenylalaine (pBoF), p-azidophenylalanine (pAzF), p-acetylphenylalanine (pAcF), p-iodophenylalanine (pIoF), p-bromophenylalanine (pBrF), p-cyanophenylalanine (pCNF), p-nitrophenylalanine (pNO2F), p-benzoylphenylalanine (pBzF), 4,4 -biphenylalanine (BIF), O-methyl-tyrosine (OMeY), O-propargyl-tyrosine (OPrY), and O-allyl-tyrosine.

growth strain :

Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	ATGAAAGCCATACGGGAAGCAATAGC
sequencing_primer_5	ACA CAT ACG ATT TAG GTG ACA CTA TAG AAT
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide

shRNA_sequence

size

2673

species

32644

Other