This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST17-UbE2D3
catalog :
15784
citations: 2
Reference
Hoppmann C, Wong A, Yang B, Li S, Hunter T, Shokat K, et al. Site-specific incorporation of phosphotyrosine using an expanded genetic code. Nat Chem Biol. 2017;13:842-844 pubmed publisher
Jin J, Li X, Gygi S, Harper J. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature. 2007;447:1135-8 pubmed
product information
Catalog Number :
15784
Product Name :
pDEST17-UbE2D3
article :
doi10.1038/nature05902
id1627
pubmed_id17597759
bacterial resistance :
Ampicillin
cloning :
backbonepDEST17
backbone_mutation
backbone_originInvitrogen
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Addgene QC NGS analysis identifies a P18L discrepancy relative to NP_003331.1. It is unknown what, if any, impact this may have on plasmid function.
origin :
37
pi :
alt_names
UbE2D3
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesE2(17)KB3, UBC4/5, UBCH5C
geneUBE2D3
id7323
genbank_ids
mutationsee Depositor Comments below
nameUbiquitin conjugating enzyme UbE2D3
shRNA_sequence
size450
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
191
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA