This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRetroSuper Smad3
catalog :
15726
citations: 6
Reference
Bryson B, Tamagno I, Taylor S, Parameswaran N, Chernosky N, Balasubramaniam N, et al. Aberrant Induction of a Mesenchymal/Stem Cell Program Engages Senescence in Normal Mammary Epithelial Cells. Mol Cancer Res. 2020;: pubmed publisher
Junk D, Bryson B, Smigiel J, Parameswaran N, Bartel C, Jackson M. Oncostatin M promotes cancer cell plasticity through cooperative STAT3-SMAD3 signaling. Oncogene. 2017;36:4001-4013 pubmed publisher
Bryson B, Junk D, Cipriano R, Jackson M. STAT3-mediated SMAD3 activation underlies Oncostatin M-induced Senescence. Cell Cycle. 2017;16:319-334 pubmed publisher
Zhou H, Chen L, Liang H, Li G, Zhang B, Chen X. Smad3 Sensitizes Hepatocelluar Carcinoma Cells to Cisplatin by Repressing Phosphorylation of AKT. Int J Mol Sci. 2016;17: pubmed publisher
Wardwell Ozgo J, Dogruluk T, Gifford A, Zhang Y, Heffernan T, Van Doorn R, et al. HOXA1 drives melanoma tumor growth and metastasis and elicits an invasion gene expression signature that prognosticates clinical outcome. Oncogene. 2014;33:1017-26 pubmed publisher
He W, Dorn D, Erdjument Bromage H, Tempst P, Moore M, Massague J. Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. Cell. 2006;125:929-41 pubmed
product information
Catalog Number :
15726
Product Name :
pRetroSuper Smad3
article :
doi10.1016/j.cell.2006.03.045
id1624
pubmed_id16751102
bacterial resistance :
Ampicillin
cloning :
backbonepRetrosuper
backbone_mutation
backbone_origin
backbone_size6400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
Smad3-shRNA-antisense: 5 -agcttttccaaaaaGGACGAGGTCTGCGTGAATtctcttgaaATTCACGCAGACCTCGTCCggg-3
-gatccccGGACGAGGTCTGCGTGAATttcaagagaATT
CACGCAGACCTCGTCCtttttggaaa-3
The following oligos were cloned into pRetrosuper-Puro digested with BglII and HindIII. Smad3-shRNA-sense: 5
origin :
37
pi :
alt_names
Smad3
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BglII
promoter
sequencing_primer_3
sequencing_primer_5H1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3
geneSMAD3
id4088
genbank_ids
mutation
nameSmad3 shRNA
shRNA_sequence
size60
species
9606
Homo sapiens
tags
resistance markers :
313
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA