This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRetroSuper-GFP Smad3
catalog :
15723
citations: 2
Reference
Oh Y, Park H, Shin J, Lee J, Park H, Kho D, et al. Ndrg1 is a T-cell clonal anergy factor negatively regulated by CD28 costimulation and interleukin-2. Nat Commun. 2015;6:8698 pubmed publisher
He W, Dorn D, Erdjument Bromage H, Tempst P, Moore M, Massague J. Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. Cell. 2006;125:929-41 pubmed
product information
Catalog Number :
15723
Product Name :
pRetroSuper-GFP Smad3
article :
doi10.1016/j.cell.2006.03.045
id1624
pubmed_id16751102
bacterial resistance :
Ampicillin
cloning :
backbonepRetrosuper-GFP
backbone_mutation
backbone_origin
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
Smad3-shRNA-antisense: 5 -agcttttccaaaaaGGACGAGGTCTGCGTGAATtctcttgaaATTCACGCAGACCTCGTCCggg-3 Next, the AgeI/XhoI fragments from shRNA sequence-containing pRetrosuper-Puro plasmids were cloned into the AgeI/XhoI site of pRetrosuper-GFP.
-gatccccGGACGAGGTCTGCGTGAATttcaagagaATT
CACGCAGACCTCGTCCtttttggaaa-3
The following oligos were first cloned into pRetrosuper-Puro digested with BglII and HindIII. Smad3-shRNA-sense: 5
origin :
37
pi :
alt_names
Smad3
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5H1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3
geneSMAD3
id4088
genbank_ids
mutation
nameSmad3 shRNA
shRNA_sequence
size450
species
9606
Homo sapiens
tags
resistance markers :
313
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA