This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2 mt-GFP (BamHI minus)
catalog :
15681
product information
Catalog Number :
15681
Product Name :
pCS2 mt-GFP (BamHI minus)
article :
doi
id1613
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCS2 mt-GFP
backbone_mutation
backbone_origin
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
xenopus/mammalian/avian/zebrafish
growth notes :
The pCS2 plasmid can be used to synthesize RNA via its SP6 promoter, or directly drive expression in eukarotic cells via the CMV promoter. In pCS2mt-GFP, the S65T mutant form of GFP has been inserted such that the myc tags and the GFP are in-frame. You can subclone your favorite sequence between the myc tags and the GFP using EcoRI and Xba I sites. The EcoRI site's AAT defines the reading frame for the insert. This produces an N-terminally myc-tagged, C-terminally GFP-tagged chimeric polypeptide. Please see lab website for more information (go to Methods -> Green Plasmids): http://spot.colorado.edu/~klym/
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name
shRNA_sequence
size
species
tags
locationN terminal on backbone
tag6x Myc
locationC terminal on backbone
tagGFP
resistance markers :
262
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA