pET14b PHAS-I | Addgene 15679 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET14b PHAS-I

catalog :

15679

citations: 2

Reference
Coffman K, Yang B, Lu J, Tetlow A, Pelliccio E, Lu S, et al. Characterization of the Raptor/4E-BP1 interaction by chemical cross-linking coupled with mass spectrometry analysis. J Biol Chem. 2014;289:4723-34 pubmed publisher
Choi K, McMahon L, Lawrence J. Two motifs in the translational repressor PHAS-I required for efficient phosphorylation by mammalian target of rapamycin and for recognition by raptor. J Biol Chem. 2003;278:19667-73 pubmed

product information

Catalog Number :

15679

Product Name :

pET14b PHAS-I

article :

doi	10.1074/jbc.M301142200
id	1612
pubmed_id	12665511

bacterial resistance :

Ampicillin

cloning :

backbone

pET14b

backbone_mutation

backbone_origin

Novagen

backbone_size

4671

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Full-length PHASI with a His tag. See author's map for detailed information.

origin :

pi :

alt_names

4EBP1

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	PHAS-I
gene	Eif4ebp1
id	116636

genbank_ids

NM_053857

mutation

Nucleotides 1-354 of rat PHAS-I plus 450bp of 3'UTR

name

PHAS-I

shRNA_sequence

size

800

species

10116

Rattus norvegicus