This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pD649-HAsp-CD8B2-Fc(DAPA)-AviTag-6xHis
catalog :
156544
citations: 1
Reference
Wojtowicz W, Vielmetter J, Fernandes R, Siepe D, Eastman C, Chisholm G, et al. A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions. Cell. 2020;182:1027-1043.e17 pubmed publisher
product information
Catalog Number :
156544
Product Name :
pD649-HAsp-CD8B2-Fc(DAPA)-AviTag-6xHis
article :
doi10.1016/j.cell.2020.07.025
id28211656
pubmed_id32822567
bacterial resistance :
Ampicillin
cloning :
backbonepD649
backbone_mutation
backbone_origin
backbone_size6106
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
growth strain :
Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
origin :
37
pi :
alt_names
CD8BL
cloning
clone_methodRestriction Enzyme
cloning_site_3GGCGCGCC
cloning_site_5GCGGCCGC
promoterCMV/SP6
sequencing_primer_3CACGTACCAGTTGAACTTCACC
sequencing_primer_5ATTTAGGTGACACTATAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD8BP
geneCD8B2
id927
genbank_ids
mutationFc region of human IgG contains D265A and P329A mutations (DAPA)
nameCD8B2
shRNA_sequence
size459
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagFc(DAPA)-AviTag-6xHis
plasmid copy :
synthesis by ThermoFisher
resistance markers :
1408
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA