This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pETM33_Orf10
catalog :
156486
product information
Catalog Number :
156486
Product Name :
pETM33_Orf10
article :
doi
id28211655
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepETM33
backbone_mutation
backbone_origin
backbone_size6017
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
insert amino acid sequence: GYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLTx
growth strain :
Bacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tag
growth temp :
Growth in BL21 DE3 gold 37 C after induction with 1mM 16hours 18 C
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5Nco1
promoterT7/LacO
sequencing_primer_3GAGTGCGGCCGCAAGCTTG
sequencing_primer_5CGGATCTGGAAGTTCTGTTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesGU280_gp11
geneORF10
id43740576
genbank_ids
mutationGene insert is codon optimized for Ecoli.
nameOrf10
shRNA_sequence
size
species
100
Synthetic
32644
Other
Severe acute respiratory syndrome coronavirus 2
tags
locationN terminal on backbone
tagHis-GST
resistance markers :
5021
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA