This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
drApo1 T7
catalog :
156461
citations: 1
Reference
Moore C, Papa L, Shoulders M. A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo. J Am Chem Soc. 2018;140:11560-11564 pubmed publisher
product information
Catalog Number :
156461
Product Name :
drApo1 T7
article :
doi10.1021/jacs.8b04001
id28211640
pubmed_id29991261
bacterial resistance :
Streptomycin
cloning :
backboneNone
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
Esherichia coli strain that expresses a deactivated variant of MutaT7, a fusion of rat APOBEC1 and the T7 RNA polymerase. Genotype: DH10B ung motAB csgABCDEFG [PlacI<>Ptac] (araA-leu)7697::[PA1lacO-Tenth drApo1-T7]
growth strain :
An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.
inserts :
MutaT7
origin :
37
pi :
alt_names
rat APOBEC1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterPA1lacO-Tenth
sequencing_primer_3
sequencing_primer_5gcagaacgcccggcac
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesREPR, apobec-1
geneApobec1
id25383
genbank_ids
mutationGlutamate 63 is mutated to Glutamine
namerApoI
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagT7 RNA polymerase
plasmid copy :
pET28b-BE1 (from David Liu addgene #73018) and pTara (from Kathleen Matthews addgene #31491)
resistance markers :
4624
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA